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【英文标准名称】:Foodstuffs-DeterminationofvitaminBbyHPLC;GermanversionENV14164:2002
【原文标准名称】:食品.采用高效液相色谱分析法测定维生素B;德文版本nENV14164:2002
【标准号】:DINVENV14164-2002
【标准状态】:现行
【国别】:德国
【发布日期】:2002-05
【实施或试行日期】:
【发布单位】:德国标准化学会(DIN)
【起草单位】:
【标准类型】:()
【标准水平】:()
【中文主题词】:食品;复合维生素B;色谱法;含量测定;维生素;高效液相色谱法;含量;测定;定量分析;分析;液相色谱法;食品检验;食品检验;化学分析和试验;高效液相色谱分析法
【英文主题词】:Analysis;Chemicalanalysisandtesting;Chromatography;Content;Determination;Determinationofcontent;Foodinspection;Foodproducts;Foodtesting;Highperformanceliquidchromatography;HPLC;Liquidchromatography;Quantitativeanalysis;VitaminBcomplex;Vitamins
【摘要】:ThisEuropeanPrestandardspecifiesamethodforthedeterminationofvitaminBinfoodstuffsbyHPLC.VitaminBisthemassfractionofthesumofpyridoxine,pyridoxal,pyridoxamineincludingtheirphosphorylatedderivativesdeterminedaspyridoxine.Theb-glycosylatedformsarenottakenintoaccount.Pyridoxal,pyridoxamineandpyridoxineareextractedfromfoodbyacidhydrolysisanddephosphorylatedenzymaticallyusingacidphosphatase.ByreactionwithglyoxylicacidinpresenceofFeascatalyst,pyridoxamineistransformedintopyridoxal,whichisthenreducedtopyridoxinebytheactionofsodiumborohydrideinalkalinemedium.PyridoxineisthenquantifiedinthesamplesolutionbyHPLCwithafluorometricdetection.infoodstuffsbyHPLC.VitaminBisthemassfractionofthesumofpyridoxine,pyridoxal,pyridoxamineincludingtheirphosphorylatedderivativesdeterminedaspyridoxine.Theb-glycosylatedformsarenottakenintoaccount.Pyridoxal,pyridoxamineandpyridoxineareextractedfromfoodbyacidhydrolysisanddephosphorylatedenzymaticallyusingacidphosphatase.ByreactionwithglyoxylicacidinpresenceofFeascatalyst,pyridoxamineistransformedintopyridoxal,whichisthenreducedtopyridoxinebytheactionofsodiumborohydrideinalkalinemedium.PyridoxineisthenquantifiedinthesamplesolutionbyHPLCwithafluorometricdetection.
【中国标准分类号】:X04
【国际标准分类号】:67_050
【页数】:13P.;A4
【正文语种】:德语